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Cell culture, Biochemistry and Molecular Biology

par douarche - publié le , mis à jour le

 

 

CELL CULTURE, BIOCHEMISTRY, AND MOLECULAR BIOLOGY


 

 

Biological samples (proteins, DNA of defined length, nucleosomes, chromatin fragments, bacteriophages etc.) are prepared in the labororatory using classical methods of biochemistry and molecular biology.

-  Over-expression of recombinant DNA and proteins (E. coli), extraction and purification
-  Reconstitiution of nucleosomes using dialysis techniques
-  Cloning and bacteria transformation (PCR, DNA engineering)

 

 

The lab is equipped with all experimental devices required for cell and bacterial culture, recombinant protein ingineering, DNA and protein purification, including :

  • HPLC
  • standard gel electrophoresis (agarose, acrylamide)
  • preparative gel electrophoresis (Prep cell Bio-Rad, FPLC)
  • incubators
  • sterile glove box and hoods
  • 4 centrifuges and 1 ultracentrifuge
  • PCR
  • 1 cold room (4°C, 9 m2)
  • 1 deep freezer (-80°C)
  • Nanodrop